Wang Z, Gerstein M, Snyder M (2009) RNA-Seq: a revolutionary tool for transcriptomics.(1995) Quantitative monitoring of gene expression patterns with a complementary DNA microarray. Bridge JA (2016) Reverse transcription-polymerase chain reaction molecular testing of cytology specimens: Pre-analytic and analytic factors.Mayer G, Müller J, Lünse CE (2011) RNA diagnostics: real-time RT-PCR strategies and promising novel target RNAs.(1988) Diagnosis of chronic myeloid and acute lymphocytic leukemias by detection of leukemia-specific mRNA sequences amplified in vitro. Kawasaki ES, Clark SS, Coyne MY et al.Gubler, U, Hoffman BJ (1983) A simple and very efficient method for generating cDNA libraries.Okayama, H, Berg P (1982) High-efficiency cloning of full-length cDNA.Lampson BC, Inouye M, Inouye S (2005) Retrons, msDNA, and the bacterial genome.Inouye S, Herzer PJ, Inouye M (1990) Two independent retrons with highly diverse reverse transcriptases in Myxococcus xanthus.Poole JC, Andrews LG, Tollefsbol TO (2001) Activity, function, and gene regulation of the catalytic subunit of telomerase (hTERT).(1997) Reconstitution of human telomerase with the template RNA component hTR and the catalytic protein subunit hTRT. (1994) An in vivo assay for the reverse transcriptase of human retrotransposon L1 in Saccharomyces cerevisiae. Dombroski BA, Feng Q, Mathias SL et al.Nobel Media AB (2014) The Nobel Prize in Physiology or Medicine 1975.Baltimore D (1970) RNA-dependent DNA polymerase in virions of RNA tumour viruses.Temin HM, Mizutani S (1970) RNA-dependent DNA polymerase in virions of Rous sarcoma virus.( Learn more about reverse transcription applications.) In addition, cDNAs serve as templates in applications such as microarray and RNA sequencing to characterize unknown RNAs in a high-throughput manner. Reverse transcription combined with PCR, or reverse transcription PCR (RT-PCR), allows detection of RNA even at very low levels of gene expression and paves the way for detection of circulating RNA, RNA viruses, and cancerous gene fusions in molecular diagnostics. These were subsequently addressed with the development of the polymerase chain reaction (PCR), a technique to amplify small amounts of genetic material. ![]() These cDNA libraries aid in understanding actively expressed genes and their functions at a specific time point.Īlthough the creation of cDNA libraries was an important step forward in characterizing expressed genes, challenges remained for the study of low-abundance RNAs. ![]() One of the first molecular biology protocols utilizing reverse transcriptases was for the production of cDNA to build libraries that contained DNA copies of mRNA from cells and tissues. While reverse transcriptases have functional roles in biological systems, they also serve as important tools for studying RNA populations.
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